STUDIES ON COMPLEMENT FIXATION TEST OF AVIAN SERUM

Studies on Complement Fixation

It is shown by complement fixation studies with protein antigens and specific immune rabbit sera that the rate of fixation of complement is determined by the concentration of antibodies in the immune sera, that the greater part of fixation of complement takes place during the 1st hour, and that fixation is practically completed at the end of 4 hours at ice box temperature. It is further shown t...

Studies on a complement fixation test for Herpes simplex.

Although the neutralization test is, in general, satisfactory for immunologic studies with a diversity of viruses, it is cumbersome and expensive. The complement fixation test, in contrast, is simple and rapid and has been successfully applied to a number of viral diseases, especially for diagnostic purposes. In the case of herpes simplex virus infections, however, the literature concerning the...

Detection of rotavirus by Latex Agglutination Test (Rotales); Comparison with Electron Microscopy and Complement Fixation Test

Quantitative micro-complement fixation test.

A quantitative micro-complement fixation test capable of detecting nanogram quantities of antigen is described. The test is simple to perform and is highly reproducible. Typical results from three antigen-antibody systems are given.

Studies on Complement Fixation in Tuberculosis. Iii

The prolonged extraction of the tubercle bacillus with boiling ethyl alcohol, followed by one or more reprecipitations by chilling the hot alcoholic solution, easily yields a preparation very active as antigen in the complement fixation reaction. This preparation gives a precipitation reaction with high dilution of the normal blood serum of a number of species. The precipitation reaction presen...